Table 3.
Multiplex PCR primers for determination of CPS type
| Mix | Product size (bp) | Penner type recognized | Gene in which primer was designed | Forward sequence | Reverse sequence |
|---|---|---|---|---|---|
| Alpha | |||||
| Mu_HS2 | 62 | HS2 | Cj1437c | CAGCATTGGAGGATTTACAATATAT | CATCCTAGCACAACTCACTTCA |
| Mu_HS3 | 149 | HS3 | HS3.17 | GGTAAGGTTGATTCTGGGTTTAAT | AGATTAGGCCAAGCAATGATAA |
| Mu_HS4 | 370 | HS4 A | HS4.07 | TATATTTGGTTAGGGATCCA | CCTAACATATCATACACTACGGT |
| Mu_HS6 | 185 | HS6 | C8J_1331 | CATACATTTGCTTTCAGATTCTTTAC | ACACGCCTATTGTTGTTGTTC |
| Mu_HS10 | 229 | HS10 | HS10.08 | TCTTATGCAGCACGCTGAT | CAAATTCAATCGACTAGCCACT |
| Mu_HS15C | 325 | HS15 and HS31 | HS15.12 | ACAGGTAATAAAATGTGCGAGTTT | ATGCATCTGCAACATCATCC |
| Mu_HS41 | 279 | HS41 | HS41.22c | CTTACATATGCTGGTAGAGATGATATG | TGCAATCTCTAAAGCCCAAG |
| Mu_HS53 | 251 | HS53 | CJE1602 | AGGCAAGCAGGAATTGTTT | TTAATTGCTCTTTGGCAATCTT |
| Beta | |||||
| Mu_HS1D | 607 | HS1 complex | HS1.08 | TTGGCGGTAAGTTTTTGAAGA | GCAAGAGAAACATCTCGCCTA |
| Mu_HS17 | 342 | HS8 and HS17 | HS17.16 | TTCACGTGGAGGATTATTGG | TTGAACATTTCATGTGTATTCCCTA |
| Mu_8486 | 652 | HS4 B | Cj8486_1475 | GTGGACATGGAACTGGGACT | AAAACGTTTAAAGTCAGTGGAAA |
| Mu_HS23 | 161 | HS23/36 | CJJ81176_1435 | GCTTGGGAGATGAATTTACCTTTA | GCTTTATATCTATCCAGTCCATTATCA |
| Mu_HS42E | 441 | HS42 | HS42.14 | ATGGTAAAACCGGCATTTCA | ATGCTTCAGTTCCACCCAAA |
| Mu_HS44 | 148 | HS44 | Not annotated | AGAAGATGCACTAGGCTCTAG | GCTATCTAATTCCATCCCTG |