Table 4.
Validation of CPS multiplex PCR with 244 strains of known Penner type
| Primer set | No. of strains with the following result: |
%a |
||||||
|---|---|---|---|---|---|---|---|---|
| Total | True positive | False positive | False negative | True negative | Accuracy | Sensitivity | Specificity | |
| HS1/44 complex | 25 | 23 | 0 | 2 | 219 | 99.18 | 92.00 | 100.00 |
| HS2 | 30 | 30 | 0 | 0 | 214 | 100.00 | 100.00 | 100.00 |
| HS3 complex | 26 | 24 | 1 | 2 | 217 | 98.77 | 92.31 | 99.54 |
| HS4 complex | 20 | 18 | 0 | 2 | 224 | 99.18 | 90.00 | 100.00 |
| HS6 | 1 | 1 | 5 | 0 | 238 | 97.95 | 100.00 | 97.94 |
| HS8/17 complex | 10 | 10 | 0 | 0 | 234 | 100.00 | 100.00 | 100.00 |
| HS10 | 14 | 13 | 2 | 1 | 228 | 98.77 | 92.86 | 99.13 |
| HS15 | 19 | 19 | 6 | 0 | 219 | 97.54 | 100.00 | 97.33 |
| HS23/36 complex | 13 | 13 | 1 | 0 | 230 | 99.59 | 100.00 | 99.57 |
| HS41 | 2 | 2 | 0 | 0 | 242 | 100.00 | 100.00 | 100.00 |
| HS42 | 8 | 8 | 0 | 0 | 236 | 100.00 | 100.00 | 100.00 |
| HS53 | 16 | 16 | 1 | 0 | 227 | 99.59 | 100.00 | 99.56 |
a
Accuracy was calculated as follows: (true positive + true negative)/(true positive + true negative + false positive + false negative). Sensitivity was calculated as follows: (true positive)/(true positive + false negative). Specificity was calculated as follows: (true negative)/(true negative + false positive).