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Ethidium bromide-stained agarose gel showing DNA amplification using suicide PCR under three different annealing temperatures (lanes 1 and 2, 54°C; lanes 3 and 4, 56°C; lanes 5 and 6, 58°C) for the groEL gene of “Candidatus Neoehrlichia mikurensis.” Lanes 1, 3, and 5, genomic DNA (gDNA) from a German dog with suspected infection; lanes 2, 4, and 6, gDNA from a healthy dog. Molecular marker, 10 kb. The 1-kb ladder is identified.
