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Limit of detection of the one-step RT-PCR tested for RABV and EBLV-1 using cell culture supernatants, brain homogenates, and saliva as biological matrices
| Species | Strain | Titer of virus stock (TCID50/ml) | Biological matrix | Sensitivity (TCID50/ml) |
|---|---|---|---|---|
| RABV | CVS-11 | 105.8 | Cell culture supernatant | 0.5 × 10−0.2 |
| Brain | 0.5 × 10−0.2 | |||
| Saliva | 0.5 × 10−0.2 | |||
| EBLV-1 | 8918FRA | 106.24 | Cell culture supernatant | 0.5 × 101.24 |
| Brain | 0.5 × 101.24 | |||
| Saliva | 0.5 × 101.24 |
