Restriction digestion of LAMP products from A. ovis. EcoRI restriction sites were introduced in FIP and BIP primers, allowing restriction fragment analysis of the specificity of the LAMP reaction. Lanes: M, DNA marker; 1, EcoRI digest of A. ovis LAMP product; 2, EcoRI enzyme activity control by digesting reconstructed plasmid DNA in pGEM-T Easy vector; 3, LAMP products from g-DNA of A. ovis.