Table 1.
Nucleotide sequences of LAMP primers for detection of A. ovis
| Primer | Type | Sequence (5′–3′)a |
|---|---|---|
| MSP4F3 | Forward outer primer | GTGTTGCACACAGATTTGCC |
| MSP4B3 | Backward outer primer | AGGCTTTTGCTTCTCCGG |
| MSP4FIP | Forward inner primer (F1c + F2) | GCCCCTGTAGGCTAGCTTTGTGgaattcCCCATATGTGTGTGCCGG |
| MSP4BIP | Backward inner primer (B1c + B2) | TGGTGGTAGGTGGGTTCTACCAgaattcATGTGCGGGTATGTCCTTG |
| MSP4LF | Loop primer F | TGTCGACAAAGCTAGCACC |
| MSP4LB | Loop primer B | CGGACTCTTTGACGAGTCTT |
a
Lowercase italics in FIP and BIP primer sequences indicate EcoRI restriction sites.