Table 2.
GyrA and ParC amino acid substitutions and detection of class 1 integrons in select Acinetobacter isolates obtained from correctional facilitiesa
| Isolate code | Species typing | FQ breakpoint |
Change in GyrA amino acid: |
Change in ParC amino acid: |
Aminoglycoside breakpoint |
Class 1 integron detection |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CIP | GAT | LVX | Gly-81 (GGT) | Ser-83 (TCA) | Ala-84 (GCT) | Glu-87 (GAA) | Ser-80 (TCG) | Glu-84 (GAA) | AMK | GEN | TOB | Presence of integrase | Cassette organization | ||
| CCF-34A | A. baumannii (clone II) | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | S | S | S | − | |
| CCF-40A | A. baumannii (clone I) | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | R | R | R | + | aacA4-catB8-aadA1 |
| CCF-43A | A. baumannii | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | S | S | S | + | |
| CCF-45A | A. baumannii (clone II) | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | R | R | R | + | aacA4-catB8-aadA1 |
| CCF-46A | A. baumannii (clone II) | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | R | R | R | + | aacA4-catB8-aadA1 |
| CCF-55A | A. baumannii | R | R | R | − | Leu (TTA) | − | − | Phe (TTT) | − | I | S | R | + | aac(6′)-Im-(aadA1/IS26/aadA1) |
| CCF-56A | A. baumannii (clone I) | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | R | R | R | − | |
| CCF-63A | A. baumannii (clone I) | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | R | R | R | + | aacA4-catB8-aadA1 |
| CCF-92A | A. baumannii | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | S | S | S | − | |
| CCF-96A | gen. sp. 3 | R | I | I | − | − | − | − | − | − | S | S | S | − | |
| CCF-104A | A. baumannii | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | S | S | S | − | |
| CCF-120A | gen. sp. 14BJ | S | S | S | − | − | − | − | Leu (TTG) | − | S | S | S | − | |
| CCF-08C | A. baumannii (clone II) | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | R | R | R | + | aacA4-catB8-aadA1 |
| CCF-09C | A. baumannii (clone II) | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | R | R | R | + | aacA4-catB8-aadA1 |
| CCF-54C | A. baumannii (clone II) | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | R | R | R | + | aacA4-catB8-aadA1 |
| CCF-61C | A. baumannii (clone II) | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | R | R | R | + | aacA4-catB8-aadA1 |
| CCF-94C | A. baumannii (clone II) | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | R | R | R | − | |
| CCF-75G | A. baumannii | R | S | S | Cys | − | − | − | − | − | S | S | S | − | |
| CCF-19H | A. baumannii | R | S | S | − | Leu (TTA) | − | − | − | − | S | I | I | − | |
| CCF-27H | A. baumannii | R | I | S | − | Leu (TTA) | − | − | − | − | S | S | S | − | |
| CCF-37I | A. baumannii (clone II) | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | S | S | S | − | |
| CCF-102I | A. baumannii | I | S | S | − | − | − | − | − | − | S | S | S | − | |
| CCF-20J | gen. sp. 14BJ | S | S | S | − | − | − | − | Leu (TTG) | − | S | S | S | − | |
| CCF-59M | A. baumannii | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | R | R | R | − | |
| CCF-70M | A. baumannii (clone II) | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | S | R | S | − | |
| CCF-82M | A. baumannii | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | S | S | S | − | |
| CCF-86M | A. baumannii | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | S | R | R | + | aadB-aadA2 |
| CCF-93M | A. baumannii (clone II) | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | S | R | R | + | aadB-aadA2 |
| CCF-108M | A. baumannii | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | S | S | S | − | |
| CCF-121M | A. baumannii | R | S | S | − | − | − | − | − | − | S | R | I | − | |
| CCF-68Q | A. baumannii | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | S | R | R | + | aadB-aadA2 |
| CCF-97Q | A. baumannii | R | I | I | − | Leu (TTA) | − | − | Leu (TTG) | − | S | R | R | + | aadB-aadA2 |
| CCF-129Q | A. baumannii | R | R | R | − | Leu (TTA) | − | − | Leu (TTG) | − | S | R | R | + | aadB-aadA2 |
Shown here are only isolates intermediate or resistant to relevant antibiotics, mutant genotype in the gyrA or parC QRDR, or positive with class 1 integrase PCR; all other isolates had identical susceptible phenotypes, were wild type in the gyrA or parC QRDR, and were negative in class 1 integrase PCR. −, No change from the wild type. Wild-type amino acids and codons are from published sequences (GenBank accession no. X82165 for gyrA and GenBank accession no. X95819 for parC). Isolates were designated susceptible (S), intermediate (I), or resistant (R) according to antibiotic breakpoint guidelines of the CLSI (10) for Acinetobacter spp. Antibiotic abbreviations: AMK, amikacin; CIP, ciprofloxacin; GAT, gatifloxacin; GEN, gentamicin; LVX, levofloxacin; and TOB, tobramycin.