Skip to main content
. 2011 Jun;49(6):2175–2182. doi: 10.1128/JCM.02270-10

Table 1.

Primers and probes used in this study

Assaya Target geneb Primer/probe sequence (5′ to 3′)c Final concn (nM) Source or reference
Influenza virus type A M Biosearch Technologies
Influenza virus type A subtype H1 HA Biosearch Technologies
Influenza virus type A subtype H3 HA Biosearch Technologies
Influenza virus type B NS Biosearch Technologies
Respiratory syncytial virus M F, GGC AAA TAT GGA AAC ATA CGT GAA 500 4
R, TCT TTT TCT AGG ACA TTG TAY TGA ACA G 250
P, FAM-CTG TGT ATG TGG AGC CTT CGT GAA GCT 50
Human parainfluenza virus type 1 HN F, AGT TGT CAA TGT CTT AAT TCG TAT CAA T 500 Internal, unpublished
R, TCG GCA CCT AAG TAA TTT TGA GTT 500
P, FAM-ATA GGC CAA AGA “T”TG TTG TCG AGA CTA TTC CAA 50
Human parainfluenza virus type 2 HN F, GCA TTT CCA ATC TAC AGG ACT ATG A 750 Internal, unpublished
R, ACC TCC TGG TAT AGC AGT GAC TGA AC 750
P, FAM-CCA TTT ACC “T”AA GTG ATG GAA TCA ATC GCA AA 50
Human parainfluenza virus type 3 HN F, TGG YTC AAT CTC AAC AAC AAG ATT TAA G 750 Internal, unpublished
R, TAC CCG AGA AAT ATT ATT TTG CC 500
P, FAM-CCC RTC TG“T” TGG ACC AGG GAT ATA CTA CAA A 200
Human metapneumovirus F F, CAA GTG TGA CAT TGC TGA YCT RAA 600 Internal, unpublished
R, ACT GCC GCA CAA CAT TTA GRA A 600
P, FAM-TGG CYG TYA GCT TCA GTC AAT TCA ACA GA 100
Rhinovirus 5′ NCR F, CPA GCC TGC GTG GC 1,000 14
R, GAA ACA CGG ACA CCC AAA GTA 1,000
P, FAM-TCC TCC GGC CCC TGA ATG YGG C 100
Enterovirus 5′ NTR F, CCT GAA TGC GGC TAA TCC 400 12
R, TTG TCA CCA TWA GCA GYC A 400
P, FAM-CCG ACT ACT TTG GGW GTC CGT GT 200
Human parechovirus 5′ NTR F, GTA ACA SWW GCC TCT GGG SCC AAA AG 400 16
R, GGC CCC WGR TCA GAT CCA YAG T 400
P, FAM-CCT RYG GGT ACC TYC WGG GCA TCC TTC 200
Pan-adenovirus Hexon F, GCC CCA GTG GTC TTA CAT GCA CAT C 500 7
R, GCC ACG GTG GGG TTT CTA AAC TT 500
P, FAM-TGC ACC AGA CCC GGG CTC AGG TAC TCC GA 100
Pan-Legionella/Legionella pneumophila 5S-23S F, GTA CTA ATT GGC TGA TTG TCT TGA CC 200 26
R, CCT GGC GAT GAC CTA CTT TCG 400
P1, FAM-ATC GTG TAA ACT CTG AC“T” CTT TAC CAA ACC TGT GG 200
P2, HEX-ATC TCG AAC TCA GAA GTG AAA C 200
Haemophilus influenzae bexA F, TGC GGT AGT GTT AGA AAA TGG TAT TAT G 600 24
R, GGA CAA ACA TCA CAA GCG GTT A 600
P, FAM-ACA AAG CGT ATC AA“T” ACT ACA ACG AGA CGC AAA AA 100
Streptococcus pneumoniae lytA F, ACG CAA TCT AGC AGA TGA AGC A 500 2
R, TCG TGC GTT TTA ATT CCA GCT 500
P, FAM-TGC CGA AAA CGC TTG ATA CAG GGA G 100
Streptococcus pyogenes spy1258 F, GCA CTC GCT ACT ATT TCT TAC CTC AA 300 CDC, Streptococcus Laboratory Protocolsd
R, GTC ACA ATG TCT TGG AAA CCA GTA AT 300
P, FAM-CCG CAA CTC ATC AAG GAT TTC TGT TAC CA 100
Mycoplasma pneumoniae ATPase F, AAG AAG CTT ATG GTA CAG GTT GGT TAA 300 Internal, unpublished
R, TGG AGG TTG GTA GCT AAG TAA GCA 900
P, FAM-TGA CTG GAA GGA “T”GT TAA GCA GGA CAA CAA ATT T 150
Chlamydophila pneumoniae MOMP F, GGG CTA TAA AGG CGT TGC TTT 500 15
R, AGA CTT TGT TCC AGT AGC TGT TGC T 500
P, FAM-CCT TGC CAA CAG ACG CTG GCG 100
Bordetella pertussis target I IS481a F, CAA GGC CGA ACG CTT CAT 300 23
R, GAG TTC TGG TAG GTG TGA GCG TAA 300
P, FAM-CAG TCG GCC TTG CGT GAG TGG G 300
Bordetella pertussis target II PtxS1 F, CGC CAG CTC GTA CTT C 700 23
R, GAT ACG GCC GGC ATT 700
P, FAM-AAT ACG TCG ACA CTT ATG GCG A 300
a

The rhinovirus, enterovirus, and Mycoplasma pneumoniae assays have been updated and will be replaced on future cards. For the Mycoplasma pneumoniae assay, see reference 25.

b

NCR, noncoding region; NTR, nontranslated region; MOMP, major outer membrane protein.

c

F, forward primer; R, reverse primer; P, probe. Quotation marks around a letter indicate an internal quencher. Underlining and boldface indicate a locked nucleic acid.