Table 1.
Assaya | Target geneb | Primer/probe sequence (5′ to 3′)c | Final concn (nM) | Source or reference |
---|---|---|---|---|
Influenza virus type A | M | Biosearch Technologies | ||
Influenza virus type A subtype H1 | HA | Biosearch Technologies | ||
Influenza virus type A subtype H3 | HA | Biosearch Technologies | ||
Influenza virus type B | NS | Biosearch Technologies | ||
Respiratory syncytial virus | M | F, GGC AAA TAT GGA AAC ATA CGT GAA | 500 | 4 |
R, TCT TTT TCT AGG ACA TTG TAY TGA ACA G | 250 | |||
P, FAM-CTG TGT ATG TGG AGC CTT CGT GAA GCT | 50 | |||
Human parainfluenza virus type 1 | HN | F, AGT TGT CAA TGT CTT AAT TCG TAT CAA T | 500 | Internal, unpublished |
R, TCG GCA CCT AAG TAA TTT TGA GTT | 500 | |||
P, FAM-ATA GGC CAA AGA “T”TG TTG TCG AGA CTA TTC CAA | 50 | |||
Human parainfluenza virus type 2 | HN | F, GCA TTT CCA ATC TAC AGG ACT ATG A | 750 | Internal, unpublished |
R, ACC TCC TGG TAT AGC AGT GAC TGA AC | 750 | |||
P, FAM-CCA TTT ACC “T”AA GTG ATG GAA TCA ATC GCA AA | 50 | |||
Human parainfluenza virus type 3 | HN | F, TGG YTC AAT CTC AAC AAC AAG ATT TAA G | 750 | Internal, unpublished |
R, TAC CCG AGA AAT ATT ATT TTG CC | 500 | |||
P, FAM-CCC RTC TG“T” TGG ACC AGG GAT ATA CTA CAA A | 200 | |||
Human metapneumovirus | F | F, CAA GTG TGA CAT TGC TGA YCT RAA | 600 | Internal, unpublished |
R, ACT GCC GCA CAA CAT TTA GRA A | 600 | |||
P, FAM-TGG CYG TYA GCT TCA GTC AAT TCA ACA GA | 100 | |||
Rhinovirus | 5′ NCR | F, CPA GCC TGC GTG GC | 1,000 | 14 |
R, GAA ACA CGG ACA CCC AAA GTA | 1,000 | |||
P, FAM-TCC TCC GGC CCC TGA ATG YGG C | 100 | |||
Enterovirus | 5′ NTR | F, CCT GAA TGC GGC TAA TCC | 400 | 12 |
R, TTG TCA CCA TWA GCA GYC A | 400 | |||
P, FAM-CCG ACT ACT TTG GGW GTC CGT GT | 200 | |||
Human parechovirus | 5′ NTR | F, GTA ACA SWW GCC TCT GGG SCC AAA AG | 400 | 16 |
R, GGC CCC WGR TCA GAT CCA YAG T | 400 | |||
P, FAM-CCT RYG GGT ACC TYC WGG GCA TCC TTC | 200 | |||
Pan-adenovirus | Hexon | F, GCC CCA GTG GTC TTA CAT GCA CAT C | 500 | 7 |
R, GCC ACG GTG GGG TTT CTA AAC TT | 500 | |||
P, FAM-TGC ACC AGA CCC GGG CTC AGG TAC TCC GA | 100 | |||
Pan-Legionella/Legionella pneumophila | 5S-23S | F, GTA CTA ATT GGC TGA TTG TCT TGA CC | 200 | 26 |
R, CCT GGC GAT GAC CTA CTT TCG | 400 | |||
P1, FAM-ATC GTG TAA ACT CTG AC“T” CTT TAC CAA ACC TGT GG | 200 | |||
P2, HEX-ATC TCG AAC TCA GAA GTG AAA C | 200 | |||
Haemophilus influenzae | bexA | F, TGC GGT AGT GTT AGA AAA TGG TAT TAT G | 600 | 24 |
R, GGA CAA ACA TCA CAA GCG GTT A | 600 | |||
P, FAM-ACA AAG CGT ATC AA“T” ACT ACA ACG AGA CGC AAA AA | 100 | |||
Streptococcus pneumoniae | lytA | F, ACG CAA TCT AGC AGA TGA AGC A | 500 | 2 |
R, TCG TGC GTT TTA ATT CCA GCT | 500 | |||
P, FAM-TGC CGA AAA CGC TTG ATA CAG GGA G | 100 | |||
Streptococcus pyogenes | spy1258 | F, GCA CTC GCT ACT ATT TCT TAC CTC AA | 300 | CDC, Streptococcus Laboratory Protocolsd |
R, GTC ACA ATG TCT TGG AAA CCA GTA AT | 300 | |||
P, FAM-CCG CAA CTC ATC AAG GAT TTC TGT TAC CA | 100 | |||
Mycoplasma pneumoniae | ATPase | F, AAG AAG CTT ATG GTA CAG GTT GGT TAA | 300 | Internal, unpublished |
R, TGG AGG TTG GTA GCT AAG TAA GCA | 900 | |||
P, FAM-TGA CTG GAA GGA “T”GT TAA GCA GGA CAA CAA ATT T | 150 | |||
Chlamydophila pneumoniae | MOMP | F, GGG CTA TAA AGG CGT TGC TTT | 500 | 15 |
R, AGA CTT TGT TCC AGT AGC TGT TGC T | 500 | |||
P, FAM-CCT TGC CAA CAG ACG CTG GCG | 100 | |||
Bordetella pertussis target I | IS481a | F, CAA GGC CGA ACG CTT CAT | 300 | 23 |
R, GAG TTC TGG TAG GTG TGA GCG TAA | 300 | |||
P, FAM-CAG TCG GCC TTG CGT GAG TGG G | 300 | |||
Bordetella pertussis target II | PtxS1 | F, CGC CAG CTC GTA CTT C | 700 | 23 |
R, GAT ACG GCC GGC ATT | 700 | |||
P, FAM-AAT ACG TCG ACA CTT ATG GCG A | 300 |
The rhinovirus, enterovirus, and Mycoplasma pneumoniae assays have been updated and will be replaced on future cards. For the Mycoplasma pneumoniae assay, see reference 25.
NCR, noncoding region; NTR, nontranslated region; MOMP, major outer membrane protein.
F, forward primer; R, reverse primer; P, probe. Quotation marks around a letter indicate an internal quencher. Underlining and boldface indicate a locked nucleic acid.