Table 2.
TLDA reproducibility
| Assaya | Within-card reproducibility (n = 14)b |
Reproducibility between cards (n = 22) |
||
|---|---|---|---|---|
| CT | % CV | CT | % CV | |
| Flu A | 21.09 | 0.69 | 21.04 | 1.05 |
| Flu A–H1 | 25.31 | 0.96 | 25.23 | 0.94 |
| Flu A–H3 | 22.88 | 1.52 | 23.02 | 1.83 |
| Flu B | 19.29 | 0.95 | 19.28 | 0.85 |
| RSV | 26.18 | 0.47 | 26.14 | 0.56 |
| HPIV1 | 24.87 | 0.36 | 24.75 | 0.69 |
| HPIV2 | 24.90 | 0.54 | 24.71 | 0.73 |
| HPIV3 | 26.62 | 0.52 | 26.61 | 0.71 |
| HMPV | 25.78 | 0.76 | 25.70 | 1.18 |
| RV | 22.77 | 0.67 | 22.69 | 0.87 |
| EV | 31.23 | 1.78 | 31.47 | 1.95 |
| HPeV | 29.72 | 4.13 | 30.19 | 4.73 |
| Pan-AdV | 21.54 | 0.96 | 21.49 | 1.13 |
| Pan-Legionella | 20.37 | 1.88 | 20.41 | 1.93 |
| L. pneu | 20.54 | 0.93 | 20.45 | 0.97 |
| H. influ | 23.25 | 0.50 | 23.24 | 0.52 |
| S. pneumo | 27.74 | 0.68 | 27.81 | 0.64 |
| S. pyo | 26.03 | 0.82 | 26.20 | 0.88 |
| M. pneumo | 17.55 | 0.34 | 17.49 | 0.46 |
| C. pneumo | 25.59 | 0.60 | 25.47 | 0.67 |
| B. pert I | 23.92 | 1.52 | 23.99 | 1.76 |
| B. pert II | 25.25 | 0.95 | 25.35 | 1.03 |
a
Flu A, influenza virus type A; Flu A–H1, influenza virus type A subtype H1; RSV, respiratory syncytial virus; HPIV1, human parainfluenza virus type 1; HMPV, human metapneumovirus; RV, rhinovirus; EV, enterovirus; HPeV, human parechovirus; pan-AdV, pan-adenovirus; L. pneu, Legionella pneumophila; H. influ, Haemophilus influenzae; S. pneumo, Streptococcus pneumoniae; S. pyo, Streptococcus pyogenes; M. pneumo, Mycoplasma pneumoniae; C. pneumo, Chlamydophila pneumoniae; B. pert I, Bordetella pertussis target I.
b
n, number of replicates; CT, threshold cycle; % CV, percent coefficient of variance.