Table 4.

Limits of detection of the TLDA and IRTP assays

Assay and targeta	LOD range		Unit of measureb	Fold decrease in TLDA LOD
	IRTP assay	TLDA
Flu A
Template A	0.6–6	6–60	EID50/ml (H1)	10
Template B	6–60	60–600	EID50/ml (H3)	10
Flu A–H1	0.6–6	6–60	EID50/ml	10
Flu A–H3	6–60	60–600	EID50/ml	10
Flu B	0.6–6	6–60	EID50/ml	10
RSV	0.6–6	60–600	Transcripts/μl	100
HPIV1	60–600	600–6000	Transcripts/μl	10
HPIV2	6–60	6–60	Transcripts/μl	1
HPIV3	6–60	60–600	Transcripts/μl	10
HMPV	600–6000	600–6000	Transcripts/μl	1
RV	0.6–6	6–60	Transcripts/μl	10
EV	6–60	60–600	Transcripts/μl	10
HPeV	0.6–6	6–60	Transcripts/μl	10
Pan-AdV
AV11	0.6–6	6–60	GE/μl (AV11)	10
AV15	6–60	600–6000	GE/μl (AV15)	100
Pan-Legionella spp.	0.06–0.6	0.6–6	GE/μl	10
L. pneu	0.06–0.6	0.6–6	GE/μl	10
H. influ	0.6–6	0.6–6	GE/μl	1
S. pneumo	0.6–6	6–60	GE/μl	10
S. pyo	6–60	60–600	GE/μl	10
M. pneumo	0.06–0.6	0.6–6	GE/μl	10
C. pneumo	0.04–0.4	4–40	IFU/ml	100
B. pert I	6–60	6–60	GE/μl	1
B. pert II	6–60	6–60	GE/μl	1

^aFlu A, influenza virus type A; Flu A–H1, influenza virus type A subtype H1; RSV, respiratory syncytial virus; HPIV1, human parainfluenza virus type 1; HMPV, human metapneumovirus; RV, rhinovirus; EV, enterovirus; HPeV, human parechovirus; pan-AdV, pan-adenovirus; AV11, adenovirus 11; L. pneu, Legionella pneumophila; H. influ, Haemophilus influenzae; S. pneumo, Streptococcus pneumoniae; S. pyo, Streptococcus pyogenes; M. pneumo, Mycoplasma pneumoniae; C. pneumo, Chlamydophila pneumoniae; B. pert I, Bordetella pertussis target I.

^bEID₅₀, 50% egg infectious dose; GE, genome equivalents; IFU, inclusion-forming units.