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. 2011 Jun;49(6):2154–2160. doi: 10.1128/JCM.02452-10

Table 2.

Mean CT of 10-fold serial dilutions of L. interrogans and L. borgpetersenii of real-time PCR assays

No. of leptospires/μl DNA concn (ng/μl) No. of gDNAb copies/μl Mean CTa
SYBR green
 TaqMan, lipL32
lfb1 secY lipL32
L. interrogans
    105 2.5 500,000 20.19 20.21 19.15 20.46
    104 0.25 50,000 23.16 23.21 22.53 22.60
    103 0.025 5,000 26.41 26.50 25.70 26.27
    102 0.0025 500 30.09 30.30 29.49 28.94
    101 0.00025 50 34.18 32.19 32.67 34.15
    100 0.000025 5 36.82 38.50 38.37 36.45
L. borgpetersenii
    105 2.5 500,000 19.92 NA 19.88 18.94
    104 0.25 50,000 22.52 NA 22.92 22.02
    103 0.025 5,000 26.11 NA 26.26 25.80
    102 0.0025 500 29.34 NA 29.38 29.31
    101 0.00025 50 33.28 NA 33.04 32.90
    100 0.000025 5 36.81 NA 36.19 36.37
a

Mean of two independent experiments done in duplicate. NA, no amplification.

b

Based on the size of the genome of L. interrogans strain Fiocruz L1-130 (4.6 Mb); 1 genome is ∼5 fg. gDNA, genomic DNA.