Table 3.
Bacterial strains used for real-time PCR assaysa
| Species | Strain | qPCR assay result |
|||
|---|---|---|---|---|---|
| SYBR green |
TaqMan, lipL32 | ||||
| lfb1 | secY | lipL32 | |||
| Pathogens | |||||
| L. interrogans | Fiocruz L1-130 | + | + | + | + |
| L. kirschneri | Moskva V | + | + | + | + |
| L. noguchii | CZ 214 K | + | + | + | + |
| L. borgpetersenii | M84 | + | + | + | + |
| L. weilii | Celledoni | + | + | + | + |
| L. santarosai | LT821 | + | + | + | + |
| L. alexanderi | L 60 | + | + | + | + |
| L. alstoniib | 79601 | + | + | + | + |
| L. kmetyi | Bejo-Iso 9 | + | − | + | + |
| Intermediates | |||||
| L. wolffii | Khorat-H2 | − | − | − | − |
| L. licerasiae | VAR010 | − | − | − | − |
| L. inadai | LT64-68 | − | − | − | − |
| L. fainei | BUT6 | − | − | − | − |
| L. broomii | 5399 | − | − | − | − |
| Saprophytes | |||||
| L. wolbachii | CDC | − | − | − | − |
| L. meyeri | Veldrat | − | − | − | − |
| L. biflexa | Patoc 1 | − | − | − | − |
| L. vanthieliic | WaZ Holland | − | − | − | − |
| L. terpstraed | LT 11-33 | − | − | − | − |
| L. yanagawaee | Sao Paulo | − | − | − | − |
| Other bacteria | |||||
| B. burgdorferi | B31 | − | − | − | − |
| B. hermsii | HS1 | − | − | − | − |
| E. coli | XL10 | − | − | − | − |
| T. denticola | ATCC 35405 | − | − | − | − |
| P. luteola | LAM | − | − | − | − |
| P. shigelloides | 24-78 | − | − | − | − |
a
Results of qPCR assays with DNA extracted from pure cultures (>2 ng/μl) and pathogenic status of Leptospira spp. are indicated.
b
Genomospecies 1.
c
Genomospecies 3.
d
Genomospecies 4.
e
Genomospecies 5.