Fig. 2.

Use of pairwise analysis for identification of three “unknown” orbiviruses. Samples are JKT-10757 from the BTV group, DPP-6031 from the Palyam virus group, and DPP6628 from the epizootic hemorrhagic disease virus (EHDV) group. A pairwise Needleman-Wunsch score for the test sequence against all other members of the database was determined. The validity of the method was confirmed by analysis of variance (ANOVA), with a comparison of the scores of sequences within species to interspecies scores. The average score (with the corresponding standard deviation, maximum, and minimum values) against the members of each genotype is plotted.