Fig. 1.

Molecular PrP^res phenotype in classical-scrapie-affected goats. (A) Western immunoblot analysis of three exemplary goats (G5, G45, and G60), with MAb P4 demonstrating a uniform PrP^res banding pattern, which is similar to those of classical sheep and goat scrapie controls (S93, G40, and C) and clearly different from that of atypical goat scrapie (G2/FS). Abbreviations: obx, obex region in the medulla oblongata; dienc, diencephalon; cbl, cerebellar cortex. Molecular mass standards are indicated on the left in kilodaltons. (B) Comparison of the molecular masses of unglycosylated (circles), monoglycosylated (squares), and diglycosylated (triangles) PrP^res moieties in classical-scrapie-affected goats of the Greek outbreak with those of classical scrapie controls and ovine BSE (ovBSE) using MAbs P4 and L42. Scores are depicted as average values, and error bars indicate standard deviations (SDs). Note that in ovine BSE the molecular mass of the unglycosylated PrP^res is ∼1 kDa lower than those for the goats under investigation and the classical scrapie controls.