Fig. 3.

Model of PrP^res fragments predicted for TSEs in small ruminants. The illustration at the top shows the full-length mature caprine prion protein and indicates the binding sites of the antibodies used for epitope mapping and the octapeptide repeat region between residues 54 and 89. The predicted PrP^res fragments for classical scrapie and ovine BSE were adopted from data described previously by Thuring and coworkers (30), while those for atypical scrapie and the aberrant Greek goats are estimated based on data from the present study. Additional glycosylated PrP^res fragments that migrate in the range of 19 to 30 kDa occur in atypical scrapie, but these are not included in this figure. The predicted N- and C-terminal residues are indicated for each fragment; gray boxes represent alternative terminal residues. To facilitate molecular mass assessments, a scale is indicated at the bottom. Glycosylation sites are shown at positions 182 and 199 (CHO). All numbered residues refer to the caprine prion protein (GenBank accession number X91999).