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Tissue autolysis and PrPres phenotype. A severely autolytic TSE-negative brain tissue macerate (Neg) was spiked with classical scrapie (Sccl1 and Sccl2) (A) and atypical scrapie (Scat1 and Scat2) (B) brain tissue macerates and subjected to further autolysis for 7 days. Samples were analyzed by NC-WB with MAb P4. Severely autolytic tissue samples from a Swiss classical sheep scrapie case (Sccl3) and an atypical goat scrapie case (S2/FS) are included as controls.
