Table 1.
PRNP genotypes at codons 211, 222, and 240 and test performances for goats that displayed an aberrant truncated PrPres by Western immunoblotting
| Animal | Age (mo) | PRNP genotyped at codon 211/222/240 | NeuroCentre Western blot result (∼12-kDa band)a |
TeSeE ODb | RDU by PrioStrip SR in 1st/2nd/3rd runc | |||
|---|---|---|---|---|---|---|---|---|
| obx | dienc | cbl | cbr | |||||
| G17 | 32 | RR/QQ/PP | + | NT | + | − | 0.023 | 51/0/0 |
| G18 | 40 | RR/QQ/PS | +++ | + | + | − | 0.023 | NT |
| G22 | 38 | RR/QQ/PS | ++ | +++ cl | − | − | 0.024 | 0 |
| G34 | 38 | RR/QK/PS | − | − | − | +++ | 0.023 | 84/0/40 |
| G50 | 24 | RR/QQ/PS | +++ | (+) | ++ | − | 0.024 | 4 |
| G53 | 36 | RQ/QQ/PS | − | − | − | + | 0.022 | 0 |
| G72 | 38 | RR/QQ/PS | + | − | − | − | 0.030 | 0 |
| G78 | 60 | RR/QK/PS | + | − | − | − | 0.022 | 97/92/150 |
| G81 | 36 | RQ/QQ/PS | − | − | − | + | 0.023 | 130/199/269 |
| G84 | 28 | RQ/QK/SS | +++ | + | + | − | 0.022 | 84/15/50 |
a
Abbreviations: obx, obex region in the medulla oblongata; dienc, diencephalon; cbl, cerebellar cortex; cbr, cerebral cortex; −, +, ++, and +++, no, weak, moderate, and strong signals, respectively; cl, classical scrapie pattern; NT, not tested. Parentheses indicate signals visible only after overexposure of the film.
b
In optical density (OD) units; cutoff, OD of 0.205 (medulla oblongata).
c
In relative density units (RDU); cutoff, 60 RDU (medulla oblongata).
d
Letters correspond to single-letter amino acid code.