Table 3.
Summary of published studies on detection of CRE from rectal swabs
| Design (n) | Method evaluateda | Results (%)b | Reference |
|---|---|---|---|
| Surveillance rectal swabs (187) | MAC plate + ERT, IMI, and MER disks; cutoff not stated | SN = 87, SP = 100 | 8 |
| blaKPC qPCR from swabs following extraction method A | SN = 100, SP = 95 | ||
| blaKPC qPCR from swabs following extraction method B | SN = 97.9, SP = 96.4 | ||
| LOD analysis of CRE strains; surveillance rectal swabs (51) | TSB + IMI disk, subcultured to MAC plates | Comparable analytical LOD, SN = 100 | 10 |
| TSB, subcultured to MAC plates + IMI disk; cutoff, <16 mm | Comparable analytical LOD, SN = 50 | ||
| Surveillance rectal swabs (149) | TSB + IMI disk, subcultured to MAC plates | SN = 65.6, SP = 49.6 | 13 |
| MAC plate + ERT disks; cutoff, <27 mm | SN = 97, SP = 90.5 | ||
| Surveillance rectal swabs (phenotypic methods compared to blaKPC PCR) (122) | MAC plate + ERT, IMI, and MER disks; cutoff not stated | SN = 92.7, SP = 95.9 | 21 |
| CHROMagar KPC | SN = 100, SP = 98.4 | ||
| Surveillance rectal swabs (755) | Inoculated BHI broth subjected to blaKPC PCR | SN = 92.2, SP = 99.4 | 22 |
| MAC + IMI (1 μg/ml) | SN = 87.5, SP = 99.6 | ||
| Surveillance rectal swabs (139) | MAC plate + ERT, IMI, and MER disks; cutoff, <22 mm | SN = 75.8, SP = 89.6 | Current study |
| MAC + IMI (1 μg/ml) | SN = 84.9, SP = 94.3 | ||
| CHROMagar KPC | SN = 84.9, SP = 88.7 |
a
TSB, tryptic soy broth; IMI, imipenem; ERT, ertapenem; MER, meropenem; MAC, MacConkey; qPCR, quantitative PCR; BHI, brain heart infusion.
b
SN, sensitivity; SP, specificity.