Table 1.
Serum PCR positivity when testing simulated serum specimens spiked with various genomic burdens
| Fungal burden (no. of ge/0.5 ml serum) | MycAssay Aspergillus PCRa |
In-house Aspergillus PCRa |
Cq difference (no. of cycles) | ||
|---|---|---|---|---|---|
| % positivity | Mean no. of Cq cycles (SD) | % positivity | Mean no. of Cq cycles (SD) | ||
| 50,000 (nd = 2) | 100 | 24.1 (0.1) | 100 | 24.4 (0.2) | 0.3 |
| 5,000 (n = 3) | 100 | 27.8 (0.5) | 100 | 28.8 (1.3) | 1.0 |
| 500 (n = 4) | 100 | 31.4 (0.3) | 100 | 33.1 (0.6) | 1.7 |
| 100 (n = 3) | 100 | 33.8 (1.6) | 100 | 35.4 (1.5) | 1.6 |
| 50 (n = 3) | 100 | 35.8 (0.5) | 100 | 38.1 (0.8) | 2.3 |
| 25 (n = 24)b | 91.6 | 35.8 (1.9) | 95.2 | 37.3 (1.6) | 1.5 |
| 25 (n = 18)c | 100 | 35.9 (2.1) | 100 | 37.1 (1.7) | 1.2 |
| 5 (n = 10)b | 80.0 | 36.8 (0.6) | 80.0 | 38.6 (1.4) | 1.7 |
| 5 (n = 7)c | 100 | 36.7 (0.5) | 100 | 38.3 (1.2) | 1.6 |
a
Results of independent testing center.
b
Data generated when testing fresh DNA and DNA template exposed to long-term storage.
c
Data generated when testing fresh DNA template only.
d
n, number of biological replicates tested at each burden. More replicates were tested at the lower burdens as, on the basis of published Cq values, these represent clinically encountered burdens.