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Sensitivity of cytb qPCR assay calculated from serial dilutions of a P. falciparum standard (NIBSC) at 9.79 parasites/100 red blood cells in blood (plain line) and in water (dashed line). Cq values are plotted against the quantity of parasites expressed as the logarithm of the parasitemia level. The plot of the mean Cq values against quantity fits a linear function (R2, >0.998).
