Table 1.

Medium formulations with and without VeroE6 cells^a

Abbreviation	Medium	Vero E6 cells	Supplementation	pH	Reference
M10-V	M199b	Present	10% fetal bovine serum, 1 mM sodium pyruvate, 10 mM HEPES, 1 μg/ml of amphotericin Bc	7.6	11
M10	M199		10% fetal bovine serum, 1 mM sodium pyruvate, 10 mM HEPES, 1 μg/ml of amphotericin B	7.6	11
S10	Schneider's		10% fetal bovine serum, 5% sucrose, 1 μg/ml of amphotericin B	7.1	22
MS10-V	40% M199b and 40% Schneider's	Present	10% fetal bovine serum, 1 mM sodium pyruvate, 10 mM HEPES, 1 μg/ml of amphotericin B	7.4	This study
MS10	40% M199b and 40% Schneider's		10% fetal bovine serum, 1 mM sodium pyruvate, 10 mM HEPES, 1 μg/ml of amphotericin B	7.4	This study

^aThe tests were repeated at both 35°C and 37°C.

^bWith Earle's salts and l-glutamine.

^cAmphotericin B is recommended when biological specimens are cultured in our laboratory.