Table 3.
Results on viruses used in this study using RT-PCR method with the oligonucleotide primers for the dual priming oligonucleotide system
| Family | Genus | Species | Straina | Resultb |
|
|---|---|---|---|---|---|
| Expected | Observed | ||||
| Bunyaviridae | Bunyavirus | Aino virus | KSA9910 | X | X |
| Orthobunyavirus | Akabane virus | 93FMX | X | X | |
| Phlebovirus | RVFV | Smithburn | O | O | |
| Flaviviridae | Flavivirus | JEV | Anyang300 | X | X |
| WNV | NY385-99 | X | X | ||
| WNV | B956 | X | X | ||
| Pestivirus | BVDV | KD26-1 | X | X | |
| BVDV | 95002 | X | X | ||
| Paramyxoviridae | Morbillivirus | CDV | Onderstepoort | X | X |
| RPV | L | O | O | ||
| RPV | LA | O | O | ||
| RPV | LATC | O | O | ||
| PPRV | Nigeria 75/1 | O | O | ||
| Picornaviridae | Aphthovirus | FMDV | O/SKR/2002 | X | X |
| Enterovirus | SVDV | UK27/72 | X | X | |
| Reoviridae | Orbivirus | BTV | RSArrr/01 (serotype 1) | O | O |
| BTV | RSArrr/02 (serotype 2) | O | O | ||
| BTV | RSArrr/03 (serotype 3) | O | O | ||
| BTV | RSArrr/04 (serotype 4) | O | O | ||
| BTV | RSArrr/05 (serotype 5) | O | O | ||
| BTV | RSArrr/06 (serotype 6) | O | O | ||
| BTV | RSArrr/07 (serotype 7) | O | O | ||
| BTV | RSArrr/08 (serotype 8) | O | O | ||
| BTV | RSArrr/09 (serotype 9) | O | O | ||
| BTV | RSArrr/10 (serotype 10) | O | O | ||
| BTV | RSArrr/11 (serotype 11) | O | O | ||
| BTV | RSArrr/12 (serotype 12) | O | O | ||
| BTV | RSArrr/13 (serotype 13) | O | O | ||
| BTV | RSArrr/14 (serotype 14) | O | O | ||
| BTV | RSArrr/15 (serotype 15) | O | O | ||
| BTV | RSArrr/16 (serotype 16) | O | O | ||
| BTV | RSArrr/17 (serotype 17) | O | O | ||
| BTV | RSArrr/18 (serotype 18) | O | O | ||
| BTV | RSArrr/19 (serotype 19) | O | O | ||
| BTV | RSArrr/20 (serotype 20) | O | O | ||
| BTV | RSArrr/21 (serotype 21) | O | O | ||
| BTV | RSArrr/22 (serotype 22) | O | O | ||
| BTV | RSArrr/23 (serotype 23) | O | O | ||
| BTV | RSArrr/24 (serotype 24) | O | O | ||
| BTV | MAY1987/01(serotype 1) | O | O | ||
| BTV | IND2003/01(serotype 2) | O | O | ||
| BTV | ISA1991/01(serotype 16) | O | O | ||
| BTV | IND1998/01(serotype 23) | O | O | ||
| BTV | IND2001/01(serotype 1) | O | O | ||
| BTV | Station (serotype 10) | O | O | ||
| BTV | 6245S (serotype 11) | O | O | ||
| BTV | 67-41B (serotype 13) | O | O | ||
| BTV | CSIRO 19 | O | O | ||
| Chuzan virus | YoungAm | X | X | ||
| EHDV-1 | NewJersey | X | X | ||
| EHDV-2 | Alberta | X | X | ||
| EHDV-2 | Ibaraki | X | X | ||
| Rhabdoviridae | Vesiculovirus | VSV | Indiana | X | X |
| Togaviridae | Alphavirus | WEEV | Unidentified | X | X |
| EEEV | Unidentified* | X | X | ||
| WEEV | Unidentified* | X | X | ||
| VEEV | Unidentified* | X | X | ||
a
*, RNAs extracted from the Cephalovac VEWT vaccine (Boehringer Ingelheim Vetmedica, St. Joseph, MO).
b
O, the multiplex RT-PCR used in this study produced amplicons of exactly the expected sizes, and they were clearly distinguishable by agarose gel electrophoresis; X, no band was observed.