Fig. 1.
NP-1 real-time PCR assay showing representative results obtained with serial 10-fold dilutions (101 to 108 copies per reaction) of the pGEM-T NP-1 HBoV2 plasmid, run in triplicate. The left panel shows baseline subtractive curve fit views of the data with relative fluorescence units (RFU) plotted against cycle number. The right panel shows a standard curve analysis of the DNA amplification plots with cycle threshold (CT) values plotted against the logarithm of the input copy number. The dynamic range of the NP-1 PCR assay spanned 8 log units with a slope of −3.3 and an R2 value of 1.0.