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. 2011 Apr;49(4):1537–1541. doi: 10.1128/JCM.00196-10

Table 3.

Reproducibility of the HBoV2 real-time PCR assay

Reproducibility type CV (%) of the no. of pGEM-T NP-1 HBoV2 plasmid DNA for the following copies/reaction:a
101 102 103 104 105 106 107 108
Intra-assayb 1.92 0.99 1.29 0.21 0.35 0.37 0.28 0.23
Interassayc 1.72 0.77 0.37 0.83 0.36 0.37 0.66 1.37
a

Tenfold serial dilutions of HBoV2 plasmid DNA are indicated. Dilution series were thawed on three different days, and assays were performed in triplicate for each dilution. CV, coefficient of variation.

b

Determined from three replicates within each assay.

c

Determined from three independent assays performed on different days.