Fig. 1.

Impact of omission of cell washes prior to biofilm formation. C. albicans DAY185 was grown overnight in YPD medium plus uridine at 30°C. Cells either were washed with sterile PBS or were directly enumerated with a hemocytometer. Biofilms were grown for 6 h and were treated with amphotericin B for 24 h. Absorbance at 492 nm was measured following incubation with XTT (0.75 mg/ml) for 1 h. Student's t test was used to compare the effects of no wash to washing at each concentration; the results were not significant.