Fig. 3.

Impact of the antifungal dosing regimen on the susceptibility of C. albicans biofilms. The reference strain DAY185 and the FKS1/fks1Δ glucan synthase mutant were grown overnight in YPD medium plus uridine at 30°C, and cells were enumerated with a hemocytometer. After a 6-h biofilm formation period, biofilms were treated with serial dilutions of fluconazole for 48 h. Fluconazole was supplied either as a single 48-h treatment (A) or as two 24-h treatments (B). Absorbance at 492 nm was measured following incubation with XTT (0.75 mg/ml) for 1 h. Student's t test was used to compare drug-treated biofilms with untreated controls. *, P < 0.05.