Fig. 4.

Impact of the XTT concentration on the susceptibility of C. albicans biofilms. The reference strain DAY185 was grown overnight in YPD medium plus uridine at 30°C, and cells were enumerated with a hemocytometer. After a 24-h biofilm formation period, biofilms were treated with serial dilutions of amphotericin B for 24 h. Absorbance at 492 nm was measured following incubation with XTT (0.25 to 1 mg/ml, as shown in the key) for 1 h. ANOVA with pairwise comparisons using the Holm-Sidak method was used to compare XTT concentrations at each drug concentration. The results were not significant.