Fig. 5.

Impact of the duration of XTT incubation on the susceptibility of C. albicans biofilms. The reference strain DAY185 was grown overnight in YPD medium plus uridine at 30°C, and cells were enumerated with a hemocytometer. After a 24-h biofilm formation period, biofilms were treated with serial dilutions of hydrogen peroxide for 24 h. Absorbance at 492 nm was measured following incubation with XTT (0.75 mg/ml) for 0.5 to 4 h. ANOVA with pairwise comparisons using the Holm-Sidak method was used to compare XTT incubation times at each drug concentration. *, P < 0.05.