Fig. 7.

Impact of omission of the XTT supernatant transfer step. The reference strain C. albicans DAY185 was grown overnight in YPD medium plus uridine at 30°C, and cells were enumerated with a hemocytometer. After a 6-h biofilm formation period, biofilms were treated with serial dilutions of amphotericin B for 24 h. XTT (0.75 mg/ml) was added, and plates were incubated for 1 h. Colorimetric absorbance was measured either directly in the 96-well plate or following the transfer of the XTT cellular supernatant to a fresh plate. Student's t test was used to compare transfer versus no transfer at each concentration. *, P < 0.05.