Fig. 8.

Biofilm treatment endpoint determination. C. parapsilosis 5986 and C. glabrata 5376 were grown overnight in YPD medium plus uridine at 30°C, and cells were enumerated with a hemocytometer. After a 24-h biofilm formation period, biofilms were treated with serial dilutions of hydrogen peroxide for 24 h. Absorbance at 492 nm was measured following incubation with XTT (0.75 mg/ml) for 1 h. Biofilm susceptibilities were compared between strains by using the EC₅₀, the percentage of reduction, and the AUC as endpoints (see Results). Student's t test was used to compare percentages of reduction between strains at each H₂O₂ concentration. *, P < 0.05.