Table 1.
Impact of subculture medium and temperature on the susceptibility of Candida biofilms
| Strain | Subculture condition |
EC50a of: |
||
|---|---|---|---|---|
| Temp (°C) | Mediumb | Amphotericin B (μg/ml) | H2O2 (mM) | |
| C. albicans DAY185 | 30 | YPD | 0.06 | 250 |
| 30 | RPMI | 0.06 | 250 | |
| 37 | YPD | NA | NA | |
| C. albicans K1 | 30 | YPD | 0.06 | 125 |
| 30 | RPMI | 0.13 | 125 | |
| 37 | YPD | 0.13 | 500 | |
| C. glabrata 5376 | 30 | YPD | 0.13 | 500 |
| 30 | RPMI | 0.06 | 1,000 | |
| 37 | YPD | 0.13 | 500 | |
| C. parapsilosis 5986 | 30 | YPD | 0.13 | 250 |
| 30 | RPMI | 0.13 | 500 | |
| 37 | YPD | 0.13 | 500 | |
a
Drug concentration associated with a 50% reduction in optical density from that for no-drug control wells. NA, not applicable.
b
YPD, yeast extract-peptone-dextrose medium (1% yeast extract, 2% peptone, 2% dextrose supplemented with uridine); RPMI, RPMI 1640 medium buffered with morpholinepropanesulfonic acid.