Table 3.

Summary of Candida biofilm microtiter plate model and XTT assay variables for experimental design

Objective	Proposed change in variables
Increase assay sensitivity	Shorten duration of biofilm formation
	Increase drug concn
	Increase antifungal treatment period
	Shorten interval between drug doses
	Shorten XTT incubation time
Increase biofilm formation	Try alternative subculture conditions (RPMI-MOPS or 37°C)
	Lengthen duration of biofilm formation
Decrease time and cost	Use cells directly from subculture without additional washes
	To avoid having to filter sterilize XTT, prepare it fresh daily
	Use XTT at 0.25 mg/ml
	Shorten XTT incubation time to 30 min
	Record absorbance directly in original 96-well plate to avoid transfer to new plate