Table 1.

Virulence genes and primers for multiplex real-time PCR for diarrheagenic E. coli^a

Diarrheagenic E. coli	Gene	Orientationb	Primer sequence (5′→3′)	Amplicon size (bp)	Amplicon Tmc (°C) (mean ± SD)
EAEC	aggR	F	CGAAAAAGAGATTATAAAAATTAAC	100	77.07 ± 0.68
		R	GCTTCCTTCTTTTGTGTAT
ETEC	st (stIa)	F	TTTCCCCTCTTTTAGTCAGTCAA	159	81.45 ± 0.27
	st (stIb)	F	TGCTAAACCAGTAGAGTCTTCAAAA	138	81.45 ± 0.27
		R	GCAGGATTACAACACAATTCACAGCAG
	lt	F	TCTCTATGTGCATACGGAGC	322	85.88 ± 0.34
		R	CCATACTGATTGCCGCAAT
EPEC	eaeA	F	ATGCTTAGTGCTGGTTTAGG	248	83.93 ± 0.31
		R	GCCTTCATCATTTCGCTTTC
STEC/EHECd	stx1	F	CTGGATTTAATGTCGCATAGTG	150	87.37 ± 0.32
		R	AGAACGCCCACTGAGATCATC
	stx2	F	GGCACTGTCTGAAACTGCTCC	255	89.65 ± 0.33
		R	TCGCCAGTTATCTGACATTCTG
EIEC	ipaH	F	GTTCCTTGACCGCCTTTCCGATACCGTC	619	91.54 ± 0.26
		R	GCCGGTCAGCCACCCTCTGAGAGTAC
DAEC	daaD	F	TGAACGGGAGTATAAGGAAGATG	444	93.81 ± 0.4
		R	GTCCGCCATCACATCAAAA

^aModified from reference 7.

^bF, forward; R, reverse.

^cT_m, melting temperature.

^dEHEC, enterohemmorhagic E. coli.