. 2011 Apr;49(4):1267–1273. doi: 10.1128/JCM.01679-10

Table 3.

Results of multiple PCR testing^a

Sample from patient V077	Initial PCR result	PCR result in:			GM ELISA result	No. of leukocytes (×1,000/μl)
Sample from patient V077	Initial PCR result	Rep. 1	Rep. 2	Rep. 3	GM ELISA result	No. of leukocytes (×1,000/μl)
1	Neg.	Neg.	Neg.	Neg.	Neg.	0
2	Neg.	Pos.	Neg.	Neg.	Neg.	0
3	Neg.	Neg.	Neg.	Neg.	Neg.	0
4	Neg.	Neg.	Neg.	Neg.	Pos.	0
5	Pos.	Pos.	Neg.	Neg.	Pos.	0
6	Neg.	Neg.	Neg.	Neg.	Pos.	0
7	Neg.	Neg.	Neg.	Neg.	Pos.	0
8	Neg.	Neg.	Neg.	Neg.	Pos.	0
9	Neg.	Neg.	Neg.	Neg.	Pos.	0
10	Pos.	Pos.	Neg.	Neg.	Neg.	0
11	Neg.	Pos.	Pos.	Pos.	Pos.	0.1
12	Neg.	Pos.	Pos.	Pos.	Pos.	0
13	Pos.	Pos.	Pos.	Pos.	Pos.	1.9
14	Neg.	Neg.	Neg.	Neg.	Pos.	6.8
15	Neg.	Neg.	Neg.	Neg.	Pos.	6.4

Samples 1 to 15 were taken with a time span of 3 to 4 days between consecutive samples. Initial results represent the first results of the Aspergillus PCR (assay 1). For all samples, PCR was repeated in triplicate (replicates [Rep.] 1 to 3) using the initially isolated DNA as the template.