Table 1.
PCR | Primer | Sequence (5′–3′) | Amplicon size (bp) |
---|---|---|---|
SV40 diagnostic | SV40-for2 | CTTTGGAGGCTTCTGGGATGCAACT | 574 |
SV40 rev | GCATGACTCAAAAAACTTAGCAATTCTG | ||
SV40-forin | CTGGTGTTGATGCAATATACTGC | 493 | |
PYV-rev | GAAAGTCTTTAGGGTCTTCTACC | ||
Type specific | SV40 ctrl out-F | ATATGCCTTTCTCATMAGAGG | 791 (classical strain 776) |
SV40 ctrl out-R | CAGACACAGTAGCAATTAGGTC | 703 (type 2 strain Ri257) | |
SV40 classic-F | CTAGAAGGTCCATTAGCTGCAAA | 666 | |
SV40 classic-R | AAACTTACCAGTTAACTTTCTGG | ||
SV40 type 2-F | AAAAGGTCCATTAATTGCAGGG | 575 | |
SV40 type 2-R | ACCTTACCTATTTGCCAGTGTTG | ||
Late region | Late-Fout | GCAAATGCAGTTAGTAGACATAC | 2,350 |
Late-Rout | TGGTCCTATAGTTAGCTAGCAC | ||
Late-Fin | GCCTCCAAAATCAGGTTGATG | 2,010 | |
Late-Rin | GGAATTCTGGCCACACTGTAG | ||
Early region | Early-Fout | GCAGTTTACTGATGACTCTCCAG | 3,450 |
Early-Rout | CTATTCTAGAAGTAGTGAGGAGGC | ||
Early-Fin | AGAATTCCATTGCCTAATTTA | 3,274 | |
Early-Rin | ATATTCCTCTTATGAGAAAGGC |