Table 1.
Primers used in PCR amplification and sequencing of Enterobius vermicularis ribosomal DNAa
| Primer | Position | Sequence (5′ to 3′) |
|---|---|---|
| TW81 | 3′ end of the 18S rDNA | GTT TCC GTA GGT GAA CCT GC |
| AB28 | 5′ end of the 28S rDNA (2812–2833) | ATA TGC TTA AGT TCA GCG GGT |
| NEMF1 | 376–393 | CGC AAA TTA CCC ACT CTC |
| S3 | 3′ end | AGT CAA ATT AAG CCG CAG |
| SSU_F_01 | 5′ end | AAC CTG GTT GAT CCT GCC AGT |
| SSU_R_26 | 927–907 | CAT TCT TGG CAA ATG CTT TCG |
| SSU_F_24 | 868–887 | AGR GGT GAA ATY CGT GGA CC |
| SSU_R_81 | 1817–1836 | TGA TCC WKC YGC AGG TTC AC |
| SSU_R_82 | 1817–1840 | TGA TCC TTC TGC AGG TTC ACC TAC |
| Ev18S.F1 | 1147–1166 | AAC ACG GGA AAA CTC ACC TG |
| Ev18S.R1 | 1341–1361 | GCA CTG ACG GTT AAG CCA AT |
| Ev18S.F2 | 445–464 | CCG GTT ATC GGA ATG AGT GG |
| Ev18S.R2 | 1024–1043 | TTC CGG AGA GCT ACC AGC TA |
| Ev18S.F3 | 963–982 | CGC CCT AGT TCT GAC CGT AA |
| Ev18S.R3 | 1539–1558 | GGA GGA TTT TCA GGG GGT TA |
a
The universal eukaryotic ITS primers TW81 and AB28 were used to amplify the ITS1-5.8S-ITS2 region; the nematode 18S consensus forward (F) primer NEMF1 was used with the universal eukaryotic reverse primer S3 (14). All small-subunit (SSU) primers were adapted from Nematode Phylogenetics Primers (http://www.nematodes.org/barcoding/sourhope/nemoprimers.html). All Ev18S primers were designed based on the 18S ribosomal DNA sequence obtained in this study. R, reverse.