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. 2011 Jul;52(7):1392–1399. doi: 10.1194/jlr.M014266

Fig. 1.

Fig. 1.

Altered dGlcT-1 expression in the fat body results in impaired energy homeostasis. A: Staining of lipid droplets that store TAG in the fat bodies of adult flies. Scale bar, 50 μm. Genotypes were as follows: control (FB-Gal4/+; +/+), UAS-dGlcT-1 (FB-Gal4/+; UAS-dGlcT-1/+), UAS-dGlcT-1 IR (FB-Gal4/+; UAS-dGlcT-1 IR/+). B: Drosophila GlcT-1 (dGlcT-1) knockdown clones (GFP-positive) harbored fewer and smaller lipid droplets (red) than controls (GFP negative). The Flp/FRT system was used to make dGlcT-1 knockdown clones in the fat body. dGlcT-1 knockdown clones were marked with GFP (hs-flp, UAS-GFP; tub-Gal4/UAS-dGlcT-1 IR; FRT82B Gal80/FRT82B). Scale bar, 50 μm. C: Measurement of whole-body TAG levels of 6 day-old male flies; n = 5 for each genotype. D, E, F: Stored carbohydrate levels were measured: glucose (D), trehalose (E), and glycogen (F); n = 8 for each genotype. The error bars represent SD.