Fig. 2.

ACC, FAS, and PEPCK mRNA expression is altered in dGlcT-1 knockdown fat bodies. A: Schematic diagram of the pathway and critical enzymes for TAG and glycerol biosynthesis. B: qRT-PCR was used to measure ACC, FAS, and PEPCK mRNAs involved in TAG and glycerol biosynthesis. FB-Gal4 was used to express UAS-dGlcT-1 IR specifically in third instar larval fat body; n = 3 for each genotype. C: Western blot analysis using anti-ACC indicated that changes in ACC mRNA expression are in good agreement with the levels of ACC enzyme protein. Proteins were collected from third instar larval fat body. The error bars represent SD.