Fig.5.

Zygotic npc1 transcripts containing SSD amino acids are essential for normal epiboly. (A) Genomic structure of zebrafish npc1. Open boxes indicate coding regions of exons. Exons shown in structure include exons 1, 2, 12, 13, and 14. Arrow indicates translational start site in exon 1. Gray shaded regions below the boxes indicate the recognition site for morpholinos used in this study. Dotted lines indicate the consequence of exon disorganization due to redirected splicing upon utilization of MO2. Red lines above the boxes denoting exons 12 and 14 indicate site of PCR primers (B) Smaller DNA fragments, due to abnormal splicing, were only visible in lanes loaded with cDNA from embryos receiving MO2. (C) The effect on epiboly following MO2 and Con MO2 injections at the one cell stage.