Fig.6.

Npc1 action in the YSL is critical for normal epiboly progression. (A, C) Schematic of injection strategies performed in this study. Injected material was either injected at the 1- or 2-cell stage (A) or at 3 hpf to target expression into the yolk and YSL nuclei (C). (B, D) Lateral views, animal pole facing upward displaying fluorescent signals at 30% epiboly in embryos co-injected with MO1 and rhodamine dextran at the 1-cell (B) or 1,000-cell stage (D). (E) The effect on epiboly following MO1 and Con MO1 injections into the YSL at the 1,000-cell stage. (F) The effect on epiboly following npc1 or tol2 mRNA injection into the YSL following Npc1 knockdown by MO1 injection at the 1- or 2-cell stage.