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Disruption of Npc1 function results in actin cytoskeleton disorganization. Lateral views of stage-matched control and MO-injected embryos. Actin filaments were labeled with Alexa Fluor 568 Phalloidin in noninjected (A) and Con MO-injected controls (B) and in embryos injected with a low dose (3.0 ng) of MO1 (C). MO1-morphant embryos revealed disorganization, loss of continuity, or clumping of actin microfilaments (C′) compared with control embryos (A′, B′).
