TABLE 1.
Epiboly Percentage Following MO1 Injections and Mouse Npc1 (mNpc1) mRNA Injections
| Clutch Number | One-way ANOVA Results | Control | 200 pg mNpc1 | 400 pg mNpc1 | 5 ng MO1 | 5 ng MO1+ 200 pg mNpc1 | 5 ng MO1+ 400 pg mNpc1 |
|---|---|---|---|---|---|---|---|
| 1 | F (5, 88) = 26.8 P < 0.001 | 69.1 ± 4.8(N = 22) | 67.2 ± 8.2d(N = 12) | 69.5 ± 4.5d(N = 10) | 47.6 ± 7.6a(N = 19) | 52.1 ± 7.2a(N = 15) | 68.4 ± 7.4b,c(N = 11) |
| 2 | F (5, 79) = 10.2 P < 0.001 | 66.4 ± 6.0(N = 16) | 65.7 ± 6.1d(N = 16) | 65.9 ± 7.0d(N = 10) | 55.7 ± 5.0a(N = 7) | 56.5 ± 4.8a(N = 21) | 67.8 ± 6.4b,c(N = 10) |
| 3 | F (5, 88) = 304.5 P < 0.001 | 84.9 ± 3.3(N = 26) | 81.3 ± 4.7d(N = 12) | 80.0 ± 4.9d(N = 7) | 39.7 ± 5.6a(N = 15) | 42.5 ± 3.8a(N = 13) | 77.6 ± 6.0b,c(N = 15) |
Top number indicates the mean level of epiboly achieved for each group (see Results for technique) ± standard deviation. Each clutch demonstrated significance at the levels shown. Data from C1 are shown in graphical form in Fig. 4G. C, clutch; N, number of embryos analyzed per group.
Posthoc analysis using Tukey's HSD indicated that these groups had significantly reduced (P < 0.001 in all cases) epiboly progression compared with control embryos.
Posthoc analysis using Tukey's HSD indicated that these groups had no significant difference when compared with controls (C1: P = 1.00; C2: P = 0.993; C3: P = 0.309).
Posthoc analysis using Tukey's HSD indicated that these groups were significantly different to embryos injected with MO1 alone (C1: P < 0.001; C2: P = 0.002; C3: P < 0.001).
Posthoc analysis using Tukey's HSD indicated that these groups were not different from control embryos (P values between 0.116 and 1.0), indicating that injection alone did not result in a change in epiboly progression.