TABLE 2.
Epiboly Percentage Following MO1 Injections and P5 treatments
| Clutch Number | One-way ANOVA Results | Control | Control + DMSO | Control + 20 µM P5 | 5 ng MO1 | 5 ng MO1 + DMSO | 5 ng MO1 + 20 µM P5 |
|---|---|---|---|---|---|---|---|
| 1 | F (5, 80) = 36.3 P < 0.001 | 86.6 ± 3.3(N = 11) | 85.9 ± 4.0(N = 11) | 86.21 ± 5.8(N = 8) | 70.7 ± 3.9a(N = 21) | 70.6 ± 5.8a(N = 12) | 77.3 ± 4.9ab(N = 19) |
| 2 | F (5, 124) = 53.6 P < 0.001 | 78.2 ± 5.0(N = 28) | 80.1± 3.9(N = 33) | 80.9 ± 5.3(N = 21) | 58.5 ± 9.2a(N = 22) | 63.3 ± 4.7a(N = 11) | 67.1 ± 8.0a,b(N = 10) |
| 3 | F (5, 114) = 58.4 P < 0.001 | 72.5 ± 3.7(N = 23) | 74.1 ± 4.7(N = 28) | 74.2 ± 4.1(N = 21) | 55.4 ± 7.8a(N = 13) | 55.9 ± 5.8a(N = 16) | 62.11 ± 4.3a,b(N = 12) |
Top number indicates the mean level of epiboly achieved for each group (see Results for technique) ± standard deviation. Each clutch demonstrated significance at the levels shown. Data from C3 are shown in graphical form in Fig. 8A. C, clutch; N, number of embryos analyzed per group.
a
Posthoc analysis using Tukey's HSD indicated that these groups had significantly reduced (P < 0.001 in all cases) epiboly progression compared with control embryos.
b
Posthoc analysis using Tukey's HSD indicated that these groups were significantly different to embryos injected with MO1 alone (C1: P = 0.001; C2: P = 0.004; C3: P = 0.013).