Fig. 2.
2. The effect of prolactin injection on Pck1 mRNA expression. Mice were treated with saline (−) or with 1 µg/g body weight of prolactin (+), and after 30 min, tissues were isolated. Pck1 mRNA expression was measured by RT-PCR and normalized with 18S rRNA in isolated mammary gland (MG) epithelial cells, isolated mammary gland adipocytes, or isolated white adipose tissue (WAT). Values are the mean ± SEM; n = 5 mice per time point.