Figure 3.
APC does not lead to increased surface expression of TF or availability of anionic phospholipids. TNFα- or control-treated cells were incubated for 3 hours with the indicated agonists. (A) Surface expression of TF was determined by immunoassay using monoclonal anti-TF. Prothrombin activation (B) and annexin V binding (C) were analyzed on the cell surface as measures of negatively charged phospholipids as described in “Reagents, antibodies, and assays.” HgCl2 was added only for 30 minutes. Agonists were removed by washing steps before the assays were performed. Means ± SEM with n = 6 are shown.