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Protection of mice (n = 6) against A. pleuropneumoniae by GST-TonB2 vaccination. Two weeks after booster vaccination the mice were challenged by intraperitoneal inoculation of either ~4.0 × 105 colony-forming units (CFU) (A) or ~1.0 × 106 CFU (B) of A. pleuropneumoniae 4074 (serovar 1). The number of surviving mice was recorded for 7 d thereafter. The protective efficacy of GST-TonB2 was significantly greater than that of GST or adjuvant alone (P < 0.01; Student’s t-test).
