FIGURE 3.

Cell proliferation of monocytic C/EBPβ-long but not C/EBPβ-short cells is inhibited, with no change in morphology. A, C/EBPβ-long and SF91 control cells were cultivated up to 96 h, and cell count analysis was performed (mean ± S.D. (error bars), n = 7). B, C/EBPβ-long and SF91 control cells were incubated up to 96 h, and ATP proliferation assays were performed (mean ± S.D., n = 3). RLU, relative light units. C, C/EBPβ-long and C/EBPβ-short cells were cultivated up to 96 h, and ATP proliferation assays were performed (mean ± S.D., n = 3). The proliferation values for SF91 cells were set as the 100% control (dashed line). D, the morphology of stably transduced THP-1 cells was analyzed 24 h after seeding using light microscopy (magnification, ×100). E, C/EBPβ-short and SF91 control cells were incubated in the presence of 50 nm PMA up to 72 h, and ATP proliferation assays were performed. The respective proliferation value at 0 h was defined as 1, and relative proliferation values were calculated (mean ± S.D., n = 3). F, C/EBPβ-short and C/EBPβ-long cells were cultivated in the presence of 50 nm PMA up to 72 h, and ATP proliferation assays were performed (mean ± S.D., n = 3). The proliferation values for PMA-stimulated SF91 cells were set as the 100% control (dashed line). The asterisks indicate statistical significance with p ≤ 0.01.