FIGURE 7.

Inhibition of proliferation by serum depletion induces macrophage morphology in C/EBPβ^KO macrophage-like cells but not in premonocytic THP-1 cells. A, C/EBPβ^KO cells were incubated in 6-well plates up to 96 h in the presence of decreasing serum concentrations (5% down to 0.5%), and cell count analysis was performed (mean ± S.D. (error bars), n = 3). B, C/EBPβ^KO cells were incubated with decreasing serum concentrations as described in A. The morphology of the cells was analyzed by light microscopy, and the amount of cells with macrophage morphology was determined at 72 h (mean ± S.D., n = 3). C, THP-1 cells were incubated up to 72 h in the presence of decreasing serum concentrations (5% down to 0.5%), and the morphology of cells was evaluated (mean ± S.D., n = 3).