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. 2011 May 5;286(26):22785–22794. doi: 10.1074/jbc.M111.233577

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Pro-BMP10 is cleaved ex vivo by endogenous furin (CHO-K1 cells), stably expressed furin (CHO-FD11/Fur cells), or transiently expressed furin (LoVo cells). Western blot (WB) analyses of 20-h conditioned medium from cells (HEK293, COS-1, CHO-K1, CHO-FD11/Fur, and CHO-FD11 cells) transiently transfected with either (ProtC)-BMP10 and an empty vector, or (ProtC)-BMP10 and the prosegment of furin (ppFurin), and from cells (LoVo cells) transfected with either empty vector, (ProtC)-BMP10 and empty vector, (ProtC)-BMP10 and furin, or (ProtC)-BMP10 and sFurin. The % of pro-BMP10 cleavage into its prosegment, calculated as prosegment/(pro-BMP10 + prosegment) × 100, is indicated below each lane along with the average % cleavage and S.D. values of several (n) independent experiments. The deficiency in endogenous PC activity of each cell line is depicted as ΔPC. Note the lack of processing of pro-BMP10 overexpressed in cell lines deficient in endogenous furin activity (CHO-FD11 and LoVo cells).