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. 2011 May 5;286(26):22785–22794. doi: 10.1074/jbc.M111.233577

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Ex vivo processing of human pro-BMP10 by overexpressed furin, PACE4, and PC5/6. Western blot (WB) analyses of 20-h conditioned media from CHO-FD11 cells transiently transfected with either empty vector (vector; lane 8), or with a vector expressing ProtC-tagged pro-BMP10 (lanes 1–7; (ProtC)-BMP10) and vectors expressing either no protein (vector), furin, PACE4, or PC5/6, or their truncated versions sFurin, PACE4-ΔC, or PC5/6-ΔC. Proteins were revealed by using a rabbit ProtC-Ab. The corresponding percentages of pro-BMP10 cleavage (%) calculated from the ratio of band intensities of prosegment/(pro-BMP10 + prosegment) are indicated. The average % of pro-BMP10 cleavage and corresponding S.D. values from 4 independent experiments (n = 4) are shown as a bar graph.